Schedule
Quiz 2 will be on Thursday, November 3 and will focus on material from Classes 14 – 18 (mostly on editing genomes, but also including most likely one question on the discussion of Quiz 1 from Class 14.)
Project reports are due Monday, 7 November. This should be a complete project report, which will be graded. You will receive feedback on it, and have an opportunity to revise and improve.
Readings
Read before Tuesday, 1 November (but earlier is better):
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DNA Book: Chapter 28: Bacterial Virus Warfare and Chapter 29: Genetic Manipulation, CRISPR & beyond
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Prarthana Mohanraju, Kira S. Makarova, Bernd Zetsche, Feng Zhang, Eugene V. Koonin, John van der Oost. Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems. Science, August 2016.
Slides and Links
Slides: Class 16: CRISPR in Bacteria
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Shigeru Iida, Markus B. Streiff, Thomas A. Bickle, and Werner Arber. Two DNA Antirestriction Systems Characterization of Bacteriophage P1, darA, and darB: Characterization of darA- Phages. Virology, 1987.
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Sydney Brenner, F. Jacob, M. Meselson. An Unstable Intermediate Carrying Information from Genes to Ribosomes for Protein Synthesis, Nature, May 1961.
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Yoshizumi Ishino, Hideo Shinagawa, Kozo Makino, Mitsuko Amemura, and Atsuo Nakata. Nucleotide Sequence of the iap Gene, Responsible for Alkaline Phosphatase Isozyme Conversion in Escherichia coli, and Identification of the Gene Product, Journal of Bacteriology, December 1987. (This is the first paper to observe the palindromic repeats in bacterial DNA.)
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Prarthana Mohanraju, Kira S. Makarova, Bernd Zetsche, Feng Zhang, Eugene V. Koonin, John van der Oost. Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems. Science, August 2016.
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Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A. Doudna, Emmanuelle Charpentier. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity, Science, 28 June 2012. [PDF]