Readings
Read before Wednesday, 2 March:
Read by the end of spring break:
- Prarthana Mohanraju, Kira S. Makarova, Bernd Zetsche, Feng Zhang, Eugene V. Koonin, John van der Oost. Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems. Science, August 2016.
Slides
The slides from the 28 February class are here: class12.pdf
Links
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Shigeru Iida, Markus B. Streiff, Thomas A. Bickle, and Werner Arber. Two DNA Antirestriction Systems Characterization of Bacteriophage P1, darA, and darB: Characterization of darA- Phages. Virology, 1987.
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Sydney Brenner, F. Jacob, M. Meselson. An Unstable Intermediate Carrying Information from Genes to Ribosomes for Protein Synthesis, Nature, May 1961.
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Yoshizumi Ishino, Hideo Shinagawa, Kozo Makino, Mitsuko Amemura, and Atsuo Nakata. Nucleotide Sequence of the iap Gene, Responsible for Alkaline Phosphatase Isozyme Conversion in Escherichia coli, and Identification of the Gene Product, Journal of Bacteriology, December 1987. (This is the first paper to observe the palindromic repeats in bacterial DNA.)
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Prarthana Mohanraju, Kira S. Makarova, Bernd Zetsche, Feng Zhang, Eugene V. Koonin, John van der Oost. Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems. Science, August 2016.
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Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A. Doudna, Emmanuelle Charpentier. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity, Science, 28 June 2012. [PDF]
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CRISPR patent: Methods and compositions for RNA-directed target DNA modification and for RNA-directed transcription modulation